LABORATORY SESSIONS

 

 

The program of the laboratory sessions, which is the main activity of the Workshop, is distributed in six different topics:

 

  • TOPIC 1: Antimicrobial susceptibility tests.
  • TOPIC 2: DNA typing of clinical isolates.
  • TOPIC 3: Hypermutation and antimicrobial resistance in P. aeruginosa.  
  • TOPIC 4: Analysis of the chromosomal resistance genes expression.
  • TOPIC 5: The contribution of b-lactamases to antimicrobial resistance.
  • TOPIC 6: The contribution of the outer membrane proteins to antimicrobial resistance.

 

 

The following list include some of the techniques that will be explained and used in the laboratory sessions:

 

·        Phenotypic analysis of resistance: disk diffusion and microdilution.

·        Isolation of chromosomal and plasmidic DNA.

·        Agarose gel electrophoresis of DNA.

·        Mutation rate.

·        Preparation of competent bacterial cells.

·        Artificial transformation assay.

·        Proteomic and Western blot analysis of cell envelopes.

·        Amplification of resistance genes by polymerase chain reaction.

·        Pulsed field gel electrophoresis and REP-PCR.

·        Spectrophotometric assay for beta-lactamase activity.

·        Detection of efflux pump expression by RT-PCR.